Using the Beckman Coulter DxFLEX flow cytometer, testing items including lymphocyte subpopulation percentage detection, HLA-B27, HLA-DR and other tests have been carried out, which can accurately and comprehensively reflect the function of the patient's immune system and help patients with abnormal immune function. Patients with ankylosing spondylitis and blood diseases can be accurately diagnosed, identified the cause, and treated in time.